Journal: bioRxiv
Article Title: From Nicotiana to apple: effector screening reveals new avenues for durable scab resistance
doi: 10.64898/2025.12.25.696553
Figure Lengend Snippet: CE proteins without the Pathogenesis-Related 1α (PR1α) signal peptide from Nicotiana tabacum were expressed in N. benthamiana leaves using Agrobacterium tumefaciens -mediated transient transformation assays (ATTAs). Total protein was extracted from leaves at 2 d post-agroinfiltration and an anti-FLAG antibody used for the immunodetection of CE proteins. The PageRuler Prestained Protein Ladder (Thermo Fisher Scientific) is shown on the left of each membrane. Asterisks (*) have been placed above CE protein bands. The expected sizes of the CE proteins are 20.4 kDa (CE28), 15.5 kDa (CE33), 21.8 kDa (CE74), 19.2 kDa (CE77), 13.5 kDa (CE89), and 22.4 kDa (CE128).
Article Snippet: The purified protein samples were resolved alongside the Precision Plus ProteinTM All Blue Prestained Protein Standard (Bio-Rad) for molecular weight estimation.
Techniques: Transformation Assay, Immunodetection, Membrane